Anti-Epoxide hydrolase/EPHX1 Antibody Picoband™

Product Name

Anti-Epoxide hydrolase/EPHX1 Antibody Picoband™

View all EPHX1 Antibodies

SKU/Catalog Number

A00899-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Epoxide hydrolase/EPHX1 Antibody Picoband™ catalog # A00899-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Epoxide hydrolase/EPHX1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00899-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Epoxide hydrolase/EPHX1 recombinant protein (Position: A42-Q455).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00899-1 is reactive to EPHX1 in Human, Mouse, Rat

Applications

A00899-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

52.949kDa

Background of EPHX1

Epoxide hydrolase 1 is an enzyme encoded by the EPHX1 gene in humans. Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Epoxide hydrolase/EPHX1 using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HCCT tissue lysates,
Lane 3: human HCCP tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epoxide hydrolase/EPHX1 antigen affinity purified polyclonal antibody (Catalog # A00899-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epoxide hydrolase/EPHX1 at approximately 53 kDa. The expected band size for Epoxide hydrolase/EPHX1 is at 53 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1).
Overlay histogram showing RT4 cells stained with A00899-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epoxide hydrolase/EPHX1 Antibody (A00899-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of 293T cells using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1).
Overlay histogram showing 293T cells stained with A00899-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epoxide hydrolase/EPHX1 Antibody (A00899-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For EPHX1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.3.2.9; EPHX; EPHX1; EPOX; Epoxide Hydratase; Epoxide Hydrolase 1; epoxide hydrolase 1, microsomal (xenobiotic); HYL1; MEH; MEH8; Microsomal Epoxide Hydrolase EPHX1 EPHX, EPOX, HYL1, MEH epoxide hydrolase 1 epoxide hydrolase 1|epoxide hydratase|epoxide hydrolase 1 microsomal|epoxide hydrolase 1, microsomal (xenobiotic)

For more info on EPHX1, check out the EPHX1 Infographic

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In this infographic, you will see the following information for EPHX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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