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Product Info Summary
| SKU: | A30709 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GTPase ERas ERAS Antibody
SKU/Catalog Number
A30709
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GTPase ERas ERAS Antibody (Catalog# A30709). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GTPase ERas ERAS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30709)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ERAS. Immunogen sequence location: 184-233
Reactive Species
A30709 is reactive to ERAS in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
25.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30709 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using ERAS Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ERAS antibody.
Specific Publications For Anti-GTPase ERas ERAS Antibody (A30709)
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5 Customer Q&As for Anti-GTPase ERas ERAS Antibody
Question
We are currently using anti-ERAS antibody A30709 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-ERAS antibody (A30709) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
I am interested in to test anti-ERAS antibody A30709 on human brain for research purposes, then I may be interested in using anti-ERAS antibody A30709 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
The products we sell, including anti-ERAS antibody A30709, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-26
Question
I was wanting to use your anti-ERAS antibody for IF for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
It shows on the product datasheet, A30709 anti-ERAS antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-14
Question
Is this A30709 anti-ERAS antibody reactive to the isotypes of ERAS?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The immunogen of A30709 anti-ERAS antibody is A peptide derived from human ERAS. Immunogen sequence location: 184-233. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-12
Question
I see that the anti-ERAS antibody A30709 works with IF, what is the protocol used to produce the result images on the product page?
K. Wu
Verified customer
Asked: 2016-03-16
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-03-16
