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Product Info Summary
| SKU: | A30676 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ERD22 KDELR2 Antibody
SKU/Catalog Number
A30676
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ERD22 KDELR2 Antibody (Catalog# A30676). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERD22 KDELR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30676)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ERD22. Immunogen sequence location: 81-130
Reactive Species
A30676 is reactive to KDELR2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
24.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30676 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using KDEL Receptor 2 Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of A549 cells, using ERD22 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC and COLO cells, using ERD22 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ERD22 KDELR2 Antibody (A30676)
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1 Customer Q&As for Anti-ERD22 KDELR2 Antibody
Question
We are currently using anti-ERD22 antibody A30676 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on bovine tissues as well?
Z. Mitchell
Verified customer
Asked: 2018-11-12
Answer
The anti-ERD22 antibody (A30676) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-12
