Anti-ERLEC1 Antibody Picoband™

Product Name

Anti-ERLEC1 Antibody Picoband™

View all ERLEC1 Antibodies

SKU/Catalog Number

A11245-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ERLEC1 Antibody Picoband™ catalog # A11245-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ERLEC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11245-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ERLEC1 recombinant protein (Position: L34-N483).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11245-1 is reactive to ERLEC1 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

55 kDa

Calculated molecular weight

Background of ERLEC1

Endoplasmic reticulum lectin 1 is a protein that in humans is encoded by the ERLEC1 gene. This gene encodes a resident endoplasmic reticulum (ER) protein that functions in N-glycan recognition. This protein is thought to be involved in ER-associated degradation via its interaction with the membrane-associated ubiquitin ligase complex. It also functions as a regulator of multiple cellular stress-response pathways in a manner that promotes metastatic cell survival. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 21.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11245-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ERLEC1 using anti-ERLEC1 antibody (A11245-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLEC1 antigen affinity purified polyclonal antibody (Catalog # A11245-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERLEC1 at approximately 55 kDa. The expected band size for ERLEC1 is at 55 kDa.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-ERLEC1 antibody (A11245-1).
Overlay histogram showing SiHa cells stained with A11245-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERLEC1 Antibody (A11245-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For ERLEC1 (Source: Uniprot.org, NCBI)

Alternative Names

C20orf30; C2orf30; CL24936; CL25084; endoplasmic reticulum lectin 1; ER lectin; erlectin 1; ERLECTIN; XTP3-B; XTP3TPBchromosome 2 open reading frame 30; XTP3-transactivated gene B protein; XTP3-transactivated protein B ERLEC1 C2orf30, CIM, CL24936, CL25084, HEL117, XTP3-B, XTP3TPB endoplasmic reticulum lectin 1 endoplasmic reticulum lectin 1|ER lectin|XTP3-transactivated gene B protein|XTP3-transactivated protein B|cancer invasion and metastasis-related|epididymis luminal protein 117|erlectin 1

For more info on ERLEC1, check out the ERLEC1 Infographic

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In this infographic, you will see the following information for ERLEC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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