Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A07267 |
|---|---|
| Size: | 200μg/vial |
| Reactive Species: | Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ERp72 Pdia4 Antibody
SKU/Catalog Number
A07267
Size
200μg/vial
Form
Liquid
Description
Boster Bio Anti-ERp72 Pdia4 Antibody (Catalog# A07267). Tested in IF, IHC-P, WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Monkey, Rabbit.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-ERp72 Pdia4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07267)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Clonality
Polyclonal
Immunogen
Synthetic peptide corresponding to aa 623-638 of mouse ERp72
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07267 is reactive to PDIA4 in Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat
Applications
A07267 is guaranteed for IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
72.932kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:1,000, colorimetric)
Detects a band of ~72kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
ERp72 was detected in paraffin-embedded sections of human cerebellum tissues using rabbit anti-ERp72 Antigen Affinity purified polyclonal antibody (Catalog # A07267) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
ERp72 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti-ERp72 Antigen Affinity purified polyclonal antibody (Catalog # A07267) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
ERp72 was detected in paraffin-embedded sections of human prostate tissues using rabbit anti-ERp72 Antigen Affinity purified polyclonal antibody (Catalog # A07267) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
ERp72 was detected in paraffin-embedded sections of human testis tissues using rabbit anti-ERp72 Antigen Affinity purified polyclonal antibody (Catalog # A07267) at 10 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
Western blot analysis of ERp72 expression in MW Marker (lane 1), rat liver microsome extract (lane 2), mouse liver microsome extract (lane 3) and human liver microsome extract (lane 4). ERp72 at 72KD was detected using rabbit anti-ERp72 Antigen Affinity purified polyclonal antibody (Catalog # A07267) at 1: 1000. The blot was developed using colorimetric method.
Protein Target Info & Infographic
Gene/Protein Information For PDIA4 (Source: Uniprot.org, NCBI)
Gene Name
PDIA4
Full Name
Protein disulfide-isomerase A4
Weight
72.932kDa
Superfamily
protein disulfide isomerase family
Alternative Names
Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ERp70; ERP70ER protein 72; ERp72; ERp-72; ERP72EC 5.3.4.1; intestinal-related); protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein; protein disulfide isomerase-associated 4; protein disulfide-isomerase A4 PDIA4 ERP70, ERP72, ERp-72 protein disulfide isomerase family A member 4 protein disulfide-isomerase A4|ER protein 70|ER protein 72|endoplasmic reticulum resident protein 70|endoplasmic reticulum resident protein 72|protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)|protein disulfide isomerase-associated 4|testicular tissue protein Li 137
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDIA4, check out the PDIA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDIA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERp72 Pdia4 Antibody (A07267)
Hello CJ!
No publications found for A07267
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ERp72 Pdia4 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-ERp72 Pdia4 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-ERp72 Pdia4 Antibody
Question
Is there a BSA free version of anti-ERp72 antibody A07267 available?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ERp72 antibody A07267 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-18
Question
See attached the WB image, lot number and protocol we used for lung muscle using anti-ERp72 antibody A07267. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-06
Question
I was wanting to use your anti-ERp72 antibody for IF for human lung muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung muscle identification?
A. Lewis
Verified customer
Asked: 2019-02-08
Answer
It shows on the product datasheet, A07267 anti-ERp72 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-08
Question
Does anti-ERp72 antibody A07267 work for IF with lung muscle?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
According to the expression profile of lung muscle, PDIA4 is highly expressed in lung muscle. So, it is likely that anti-ERp72 antibody A07267 will work for IF with lung muscle.
Boster Scientific Support
Answered: 2018-10-30
Question
My question regarding product A07267, anti-ERp72 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07267 anti-ERp72 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-05
Question
We are currently using anti-ERp72 antibody A07267 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The anti-ERp72 antibody (A07267) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-10
