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Product Info Summary
| SKU: | M15907 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ERVK-7 Antibody (N-Term)
SKU/Catalog Number
M15907
Size
50 µl
Description
Boster Bio Anti-ERVK-7 Antibody (N-Term) (Catalog # M15907). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ERVK-7 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M15907)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ERVK-7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-128 amino acids from human ERVK-7.
Reactive Species
M15907 is reactive to ERVK-7 in Human
Calculated molecular weight
66.6 kDa
Background of ERVK-7
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M15907 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: human lung lysate
Lane 3: human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 67 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M15907 staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-ERVK-7 Antibody (N-Term) (M15907)
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