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Product Info Summary
| SKU: | M08004 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Esrp-2 Monoclonal Antibody
SKU/Catalog Number
M08004
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Esrp-2 Monoclonal Antibody (Catalog # M08004). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Esrp-2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08004)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 22C5.H7.A6 IgG2a kappa
Isotype
IgG2a kappa
Immunogen
Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Reactive Species
M08004 is reactive to ESRP2 in Mouse
Calculated molecular weight
77.4 kDa
Background of ESRP2
Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08004 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:100,000
WB: 1:1000
Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Validation Images & Assay Conditions
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Anti-ESRP2 by western blot shows detection of ESRP2 in lane 2. Lane G: GFP-transfected-293T cell extract, Lane 1: ESRP1-transfected 293T cell extract, Lane 2: ESRP2-transfected 293T cell extract. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.
Specific Publications For Anti-Esrp-2 Monoclonal Antibody (M08004)
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7 Customer Q&As for Anti-Esrp-2 Monoclonal Antibody
Question
We are currently using anti-Esrp-2 Monoclonal antibody M08004 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The anti-Esrp-2 Monoclonal antibody (M08004) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-13
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Esrp-2 Monoclonal antibody M08004. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
Is a blocking peptide available for product anti-Esrp-2 Monoclonal antibody (M08004)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do provide the blocking peptide for product anti-Esrp-2 Monoclonal antibody (M08004). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-13
Question
I was wanting to use your anti-Esrp-2 Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
It shows on the product datasheet, M08004 anti-Esrp-2 Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-18
Question
Would M08004 anti-Esrp-2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Anderson
Verified customer
Asked: 2018-01-09
Answer
It shows on the product datasheet, M08004 anti-Esrp-2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-Esrp-2 Monoclonal antibody M08004. Please let me know if you require anything else.
C. Parker
Verified customer
Asked: 2016-01-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-11
Question
My question regarding product M08004, anti-Esrp-2 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Thomas
Verified customer
Asked: 2014-06-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08004 anti-Esrp-2 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-18
