Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband™

Product Name

Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband™

View all ERR gamma/NR3B3 Antibodies

SKU/Catalog Number

A01470-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband™ catalog # A01470-1. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01470-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ESRRG recombinant protein (Position: Q302-V458).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01470-1 is reactive to ESRRG in Human, Mouse, Rat

Applications

A01470-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

51.306kDa

Background of ERR gamma/NR3B3

Estrogen-related receptor gamma (ERR-gamma), also known as NR3B3, is a nuclear receptor that in humans is encoded by the ESRRG gene. This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ESRRG using anti-ESRRG antibody (A01470-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates,
Lane 2: human 22RV1 whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified polyclonal antibody (Catalog # A01470-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51KD. The expected band size for ESRRG is at 51KD.

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Figure 2. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1).
ESRRG was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1).
ESRRG was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IF analysis of ESRRG using anti-ESRRG antibody (A01470-1).
ESRRG was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 5. Flow Cytometry analysis of U20S cells using anti-ESRRG antibody (A01470-1).
Overlay histogram showing U20S cells stained with A01470-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ESRRG Antibody (A01470-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ESRRG (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781L1617; ERR gamma; ERR gamma-2; ERR3; ERRG2; ERRgamma; ESRRG; Estrogen receptor-related protein 3; estrogen-related receptor gamma; FLJ16023; KIAA0832; NR3B3; Nuclear receptor subfamily 3 group B member 3 ESRRG ERR-gamma, ERR3, ERRg, ERRgamma, NR3B3 estrogen related receptor gamma estrogen-related receptor gamma|ERR gamma-2|estrogen receptor-related protein 3|nuclear receptor subfamily 3 group B member 3

For more info on ESRRG, check out the ESRRG Infographic

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In this infographic, you will see the following information for ESRRG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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