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Product Info Summary
| SKU: | EK1170 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Tenascin-C/TNC ELISA Kit PicoKine®
SKU/Catalog Number
EK1170
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Tenascin-C/TNC ELISA Kit PicoKine® (96 Tests). Quantitate Human TNC in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Tenascin-C/TNC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1170)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G23-P625
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1170 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1170 is reactive to TNC in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1170 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNC
Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1170-CAP | Anti-Human TNC Pre-coated 96-well strip microplate | 1 |
| EK1170-ST | Human TNC Standard | 2 vials, 10 ng/tube |
| EK1170-DA | Human TNC Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.001 | 0.023 | 0.052 | 0.109 | 0.235 | 0.512 | 1.033 | 1.887 |
Data Example Images
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Human Tenascin-C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNC, Dilution ratio of 1:100, concentration in serum and plasma is around 80 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Tenascin-C/TNC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 94 | 868 | 3019 | 89 | 892 | 3055 |
| Standard deviation | 4.51 | 61.62 | 223.4 | 5.51 | 74.03 | 226.07 |
| CV (%) | 4.8% | 7.1% | 7.4% | 6.2% | 8.3% | 7.4% |
Specific Publications For Human Tenascin-C/TNC ELISA Kit PicoKine® (EK1170)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human Tenascin-C/TNC ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for Tenascin C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-08-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tenascin C for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-08-11
Question
Q: What is the optimal O.D. value for Tenascin C ELISA kit? I used your Tenascin C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Tenascin C even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Tenascin C ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Tenascin C that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-03-27
Question
Which antibodies are used as capture and detector antibody for EK1170 (host species, mono- or polyclonal)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
For the Human Tenascin-C/TNC ELISA Kit PicoKine™ (EK1170), capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat.
Boster Scientific Support
Answered: 2020-03-05
Question
Q: how to proceed with the analysis of ELISA data? I have performed Tenascin C level in serum.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-02-13
Question
Do you have experience with liquor (CSF samples) using EK1170? Any recommendations?
Verified customer
Asked: 2019-03-05
Answer
Our lab technicians haven't tested the Human Tenascin-C/TNC ELISA Kit PicoKine™ (EK1170) in liquor (CSF samples). We suggest the customer to run a pilot test. In theory, the kit is able to detect the target protein if the expression level of TNC in the CSF sample falls in the assay range of EK1170.
Boster Scientific Support
Answered: 2019-03-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Jones
Verified customer
Asked: 2017-10-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-10-19
