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Product Info Summary
| SKU: | A00931-5 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-ETS1 Antibody (N-term)
SKU/Catalog Number
A00931-5
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-ETS1 Antibody (N-term) (Catalog # A00931-5). Tested in WB, IF, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ETS1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00931-5)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00931-5 is reactive to ETS1 in Human
Calculated molecular weight
50.4 kDa
Background of ETS1
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00931-5 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of ETS1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-ETS1 Antibody (N-term) at 1:1000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: 293T whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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ETS1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-ETS1 Antibody (N-term) (A00931-5)
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