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Product Info Summary
| SKU: | A13162 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EXOSC6 Antibody (N-term)
SKU/Catalog Number
A13162
Size
80 µl
Description
Boster Bio Anti-EXOSC6 Antibody (N-term) (Catalog # A13162). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EXOSC6 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13162)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EXOSC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-38 amino acids from the N-terminal region of human EXOSC6.
Reactive Species
A13162 is reactive to EXOSC6 in Human
Calculated molecular weight
28.2 kDa
Background of EXOSC6
This gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13162 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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All lanes : Anti-EXOSC6 Antibody (N-term) at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: WiDr whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 28 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-EXOSC6 Antibody (N-term) (A13162)
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