Product Info Summary
SKU: | A01750 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Ezrin Antibody
SKU/Catalog Number
A01750
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Ezrin Antibody (Catalog # A01750). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ezrin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01750)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Ezrin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01750 is reactive to EZR in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
Calculated molecular weight
69413 MW
Background of Ezrin
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Lixin Zhu, Am J Physiol Cell Physiol, Jun 2005; 288: C1242 - C1254.
H.J. Spence, Hum. Mol. Genet., Aug 2004; 13: 1657 - 1668.
Vera L¨²cia Bonilha, J. Cell Biol., Dec 1999; 147: 1533.
M Berryman, J. Cell Biol., Dec 1995; 131: 1231.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01750 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Positive Control
Validation Images & Assay Conditions
![a01750 western blotting analysis 1 a01750 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a01750-western-blotting-analysis-1.jpg)
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Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using Ezrin antiobdy A01750. The lane on the left is treated with synthesized peptide.
![a01750 western blotting analysis 2 a01750 western blotting analysis 2](https://www.bosterbio.com/media/catalog/product/a/0/a01750-western-blotting-analysis-2.jpg)
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Western blot analysis of extracts from NIH/3T3 cells, using Ezrin antibody A01750.
![a01750 immunohistochemistry analysis 1 a01750 immunohistochemistry analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a01750-immunohistochemistry-analysis-1.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ezrin antibody A01750.
Protein Target Info & Infographic
Gene/Protein Information For EZR (Source: Uniprot.org, NCBI)
Gene Name
EZR
Full Name
Ezrin
Weight
69413 MW
Alternative Names
CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; EZR; Ezrin; FLJ26216; p81; VIL2; VIL2MGC1584; villin 2 (ezrin); Villin 2; villin-2 EZR CVIL, CVL, HEL-S-105, VIL2 ezrin ezrin|cytovillin 2|epididymis secretory protein Li 105|p81|villin 2 (ezrin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EZR, check out the EZR Infographic
![EZR infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EZR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ezrin Antibody (A01750)
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No publications found for A01750
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