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Product Info Summary
| SKU: | M00562 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Factor H CFH Monoclonal Antibody
SKU/Catalog Number
M00562
BM5246 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Factor H CFH Monoclonal Antibody catalog # M00562. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor H CFH Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00562)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACCI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Factor H Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway.
Reactive Species
M00562 is reactive to CFH in Human
Observed Molecular Weight
180 kDa
Calculated molecular weight
139.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00562 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (M00562).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified monoclonal antibody (M00562) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 180 kDa. The expected band size for Factor H/CFH is at 139 kDa.
Specific Publications For Anti-Factor H CFH Monoclonal Antibody (M00562)
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6 Customer Q&As for Anti-Factor H CFH Monoclonal Antibody
Question
What is the immunogen sequence of M00562?
Verified customer
Asked: 2020-10-09
Answer
The immunogen sequence of the Anti-Factor H CFH Monoclonal Antibody (M00562) is aa161-174.
Boster Scientific Support
Answered: 2020-10-12
Question
See attached the WB image, lot number and protocol we used for liver using anti-Factor H Monoclonal antibody M00562. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-19
Question
What synthetic peptide sequence was used to generate M00562?
Verified customer
Asked: 2020-01-17
Answer
The synthetic peptide sequence of the Anti-Factor H CFH Monoclonal Antibody (M00562) is CAMEPDREYHFGQAV.
Boster Scientific Support
Answered: 2020-01-21
Question
Does M00562 react to any of the CFHR proteins (1-5), or CFHL1?
Verified customer
Asked: 2020-01-17
Answer
Cross reactivity with CFHR proteins (1-5), or CFHL1 of the Anti-Factor H CFH Monoclonal Antibody (M00562) hasn't been experimentally validated.
Boster Scientific Support
Answered: 2020-01-21
Question
My lab would like to test anti-Factor H Monoclonal antibody M00562 on human liver for research purposes, then I may be interested in using anti-Factor H Monoclonal antibody M00562 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The products we sell, including anti-Factor H Monoclonal antibody M00562, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-Factor H Monoclonal antibody M00562 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-Factor H Monoclonal antibody (M00562) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
