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Product Info Summary
| SKU: | A00440-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Factor V/F5 Antibody Picoband®
SKU/Catalog Number
A00440-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor V/F5 Antibody Picoband® catalog # A00440-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor V/F5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00440-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Factor V.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00440-1 is reactive to F5 in Human
Observed Molecular Weight
252 kDa
Calculated molecular weight
251.7 kDa
Background of F5
Factor V is a protein of the coagulation system, rarely referred to as proaccelerin or labile factor. The gene for factor V is located on the first chromosome (1q23). This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00440-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Positive Control
WB: human plasma
Validation Images & Assay Conditions
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Western blot analysis of Factor V using anti-Factor V antibody (A00440-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human plasma lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor V antigen affinity purified polyclonal antibody (Catalog # A00440-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor V at approximately 252KD. The expected band size for Factor V is at 252KD.
Specific Publications For Anti-Factor V/F5 Antibody Picoband® (A00440-1)
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Customer Q&As
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4 Customer Q&As for Anti-Factor V/F5 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Factor V/F5 antibody (A00440-1)?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
We do provide the blocking peptide for product anti-Factor V/F5 antibody (A00440-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-Factor V/F5 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can see on the product datasheet, A00440-1 anti-Factor V/F5 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-13
Question
We are currently using anti-Factor V/F5 antibody A00440-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The anti-Factor V/F5 antibody (A00440-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-03
Question
Is there a BSA free version of anti-Factor V/F5 antibody A00440-1 available?
J. Wu
Verified customer
Asked: 2015-12-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Factor V/F5 antibody A00440-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-24
