Anti-FAKD3 FASTKD3 Antibody

FAKD3 antibody

Boster Bio Anti-FAKD3 FASTKD3 Antibody (Catalog # A14273). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A14273
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-FAKD3 FASTKD3 Antibody

View all FAKD3 Antibodies

SKU/Catalog Number

A14273

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-FAKD3 FASTKD3 Antibody (Catalog # A14273). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FAKD3 FASTKD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14273)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human FAKD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14273 is reactive to FASTKD3 in Human

Reconstitution

Calculated molecular weight

75689 MW

Background of FAKD3

This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants.

The MGC Project Team, Genome Res. 14:2121-2127(2004).
Sjoeblom T., Science 314:268-274(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14273 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For FASTKD3 (Source: Uniprot.org, NCBI)

Gene Name

FASTKD3

Full Name

FAST kinase domain-containing protein 3, mitochondrial

Weight

75689 MW

Superfamily

FAST kinase family

Alternative Names

FAST kinase domain-containing protein 3, mitochondrial;FASTKD3;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FASTKD3, check out the FASTKD3 Infographic

FASTKD3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FASTKD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-FAKD3 FASTKD3 Antibody

Question

I see that the anti-FAKD3 antibody A14273 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-03

Question

I have a question about product A14273, anti-FAKD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14273 anti-FAKD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-25

Question

Would A14273 anti-FAKD3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Wu

Verified customer

Asked: 2018-12-05

Answer

It shows on the product datasheet, A14273 anti-FAKD3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-05

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-FAKD3 antibody A14273. Let me know if you need anything else.

J. Brown

Verified customer

Asked: 2018-03-08

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-08

Question

Is a blocking peptide available for product anti-FAKD3 antibody (A14273)?

B. Moore

Verified customer

Asked: 2015-10-14

Answer

We do provide the blocking peptide for product anti-FAKD3 antibody (A14273). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-10-14

Order DetailsPrice
A14273-100uL

100uL

$360

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A14273
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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