Product Info Summary
SKU: | A14273 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-FAKD3 FASTKD3 Antibody
SKU/Catalog Number
A14273
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FAKD3 FASTKD3 Antibody (Catalog # A14273). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAKD3 FASTKD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14273)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FAKD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14273 is reactive to FASTKD3 in Human
Reconstitution
Calculated molecular weight
75689 MW
Background of FAKD3
This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Sjoeblom T., Science 314:268-274(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14273 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using FAKD3 antibody A14273.
Protein Target Info & Infographic
Gene/Protein Information For FASTKD3 (Source: Uniprot.org, NCBI)
Gene Name
FASTKD3
Full Name
FAST kinase domain-containing protein 3, mitochondrial
Weight
75689 MW
Superfamily
FAST kinase family
Alternative Names
FAST kinase domain-containing protein 3, mitochondrial;FASTKD3;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FASTKD3, check out the FASTKD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FASTKD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAKD3 FASTKD3 Antibody (A14273)
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No publications found for A14273
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-FAKD3 FASTKD3 Antibody
Question
I see that the anti-FAKD3 antibody A14273 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A14273, anti-FAKD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14273 anti-FAKD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
Would A14273 anti-FAKD3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Wu
Verified customer
Asked: 2018-12-05
Answer
It shows on the product datasheet, A14273 anti-FAKD3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-FAKD3 antibody A14273. Let me know if you need anything else.
J. Brown
Verified customer
Asked: 2018-03-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-08
Question
Is a blocking peptide available for product anti-FAKD3 antibody (A14273)?
B. Moore
Verified customer
Asked: 2015-10-14
Answer
We do provide the blocking peptide for product anti-FAKD3 antibody (A14273). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-14