Anti-FAM29A/HAUS6 Antibody Picoband®

Western blot analysis of FAM29A/HAUS6 using anti-FAM29A/HAUS6 antibody (A10580-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM29A/HAUS6 antigen affinity purified polyclonal antibody (A10580-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM29A/HAUS6 at approximately 109 kDa. The expected band size for FAM29A/HAUS6 is at 109 kDa.

IF analysis of FAM29A/HAUS6 using anti-FAM29A/HAUS6 antibody (A10580-1) and anti-Beta Tubulin antibody (M01857-3).
FAM29A/HAUS6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FAM29A/HAUS6 Antibody (A10580-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunoprecipitating FAM29A/HAUS6 in MCF-7 whole cell lysate.
Western blot analysis of FAM29A/HAUS6 using anti-FAM29A/HAUS6 antibody (A10580-1);
Lane 1: MCF-7 whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-FAM29A/HAUS6 antibody in MCF-7 whole cell lysate;
Lane 3: anti-FAM29A/HAUS6 antibody (2μg) + MCF-7 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAM29A/HAUS6 antigen affinity purified polyclonal antibody (A10580-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for FAM29A/HAUS6 at approximately 109 kDa. The expected band size for FAM29A/HAUS6 is at 109 kDa.