Product Info Summary
SKU: | A17953 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-FAM35A/B Antibody
SKU/Catalog Number
A17953
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FAM35A/B Antibody (Catalog # A17953). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAM35A/B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17953)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FAM35A/B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A17953 is reactive to SHLD2 in Human
Applications
A17953 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
93.705kDa
Background of SHLD2
Belongs to the FAM35 family. 2 isoforms of the human protein are produced by alternative splicing.
Deloukas P., Nature 429:375-381(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using FAM35A/B antibody A17953.
Protein Target Info & Infographic
Gene/Protein Information For SHLD2 (Source: Uniprot.org, NCBI)
Gene Name
SHLD2
Full Name
Shieldin complex subunit 2
Weight
93.705kDa
Superfamily
SHLD2 family
Alternative Names
Shieldin complex subunit 2 SHLD2 FAM35A, FAM35A1, RINN2, bA163M19.1 shieldin complex subunit 2 shieldin complex subunit 2|RINN1-REV7-interacting novel NHEJ regulator 2|family with sequence similarity 35 member A|protein FAM35A|shield complex subunit 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHLD2, check out the SHLD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHLD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAM35A/B Antibody (A17953)
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No publications found for A17953
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-FAM35A/B Antibody
Question
We are currently using anti-FAM35A/B antibody A17953 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-FAM35A/B antibody (A17953) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
I see that the anti-FAM35A/B antibody A17953 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-17
Question
Is there a BSA free version of anti-FAM35A/B antibody A17953 available?
A. Rodriguez
Verified customer
Asked: 2018-01-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-FAM35A/B antibody A17953 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-12
Question
My question regarding product A17953, anti-FAM35A/B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17953 anti-FAM35A/B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-30