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Product Info Summary
| SKU: | A03129 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Fanconi anemia group G protein FANCG Antibody
SKU/Catalog Number
A03129
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Fanconi anemia group G protein FANCG Antibody (Catalog # A03129). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Fanconi anemia group G protein FANCG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03129)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region near amino acids 1-25 of human FANCG protein.
Reactive Species
A03129 is reactive to FANCG in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
68.6 kDa
Background of FANCG
FANCG (also called Protein FACG or DNA-repair protein XRCC9) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCG may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. This protein may also function as a tumor suppressor gene. FANCG belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. FANCG is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is highly expressed in testis and thymus and is also found in lymphoblasts. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03129 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 69 kDa in size corresponding to FANCG by western blotting in the appropriate human tissue.
Validation Images & Assay Conditions
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Western Blot of Rabbit anti-FANCG antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lysate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 10 µg per lane. Primary antibody: FANCG antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 69 kDa for FANCG.
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Boster's Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Western blot using Boster's affinity purified anti-FANCG antibody shows detection of a band at ~69 kDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate . Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-Fanconi anemia group G protein FANCG Antibody (A03129)
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5 Customer Q&As for Anti-Fanconi anemia group G protein FANCG Antibody
Question
Is this A03129 anti-FANCG antibody reactive to the isotypes of FANCG?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The immunogen of A03129 anti-FANCG antibody is This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1-12 of human FANCG protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-13
Question
Please see the WB image, lot number and protocol we used for kidney uterus using anti-FANCG antibody A03129. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney uterus using anti-FANCG antibody A03129. Let me know if you need anything else.
D. Krishna
Verified customer
Asked: 2019-05-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-FANCG antibody A03129 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
The anti-FANCG antibody (A03129) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-05
Question
I was wanting to use your anti-FANCG antibody for WB for human kidney uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human kidney uterus identification?
H. Brown
Verified customer
Asked: 2018-01-17
Answer
As indicated on the product datasheet, A03129 anti-FANCG antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human kidney uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-17
