Anti-Phospho-FANCI (Ser556) Antibody

Product Name

Anti-Phospho-FANCI (Ser556) Antibody

View all FANCI Antibodies

SKU/Catalog Number

P05108

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-FANCI (Ser556) Antibody (Catalog # P05108). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-FANCI (Ser556) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05108)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P05108 is reactive to FANCI in Human

Applications

P05108 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

149.324kDa

Background of FANCI

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitination by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser-556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser⁵⁵⁶ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase, 800 units/1mg protein for 30 min).

Gene/Protein Information For FANCI (Source: Uniprot.org, NCBI)

Alternative Names

Fanconi anemia group I protein; Fanconi anemia, complementation group I; KIAA1794FLJ10719; Protein FACI FANCI KIAA1794 FA complementation group I Fanconi anemia group I protein|Fanconi anemia complementation group I

For more info on FANCI, check out the FANCI Infographic

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In this infographic, you will see the following information for FANCI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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