Anti-FATP3/SLC27A3 Antibody Picoband™

Product Name

Anti-FATP3/SLC27A3 Antibody Picoband™

View all FATP3/SLC27A3 Antibodies

SKU/Catalog Number

A12345-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FATP3/SLC27A3 Antibody Picoband™ catalog # A12345-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-FATP3/SLC27A3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12345-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FATP3/SLC27A3 recombinant protein (Position: L192-I683).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12345-1 is reactive to SLC27A3 in Human, Mouse

Applications

A12345-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

70-79 kDa

Calculated molecular weight

73.55kDa

Background of FATP3/SLC27A3

Long-chain fatty acid transport protein 3 is a protein that in humans is encoded by the SLC27A3 gene. This gene belongs to a family of integral membrane proteins and encodes a protein that is involved in lipid metabolism. The increased expression of this gene in human neural stem cells derived from induced pluripotent stem cells suggests that it plays an important role in early brain development. Naturally occurring mutations in this gene are associated with autism spectrum disorders. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP3/SLC27A3 antigen affinity purified polyclonal antibody (Catalog # A12345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP3/SLC27A3 at approximately 70-79 kDa. The expected band size for FATP3/SLC27A3 is at 74 kDa.

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Figure 2. IHC analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1).
FATP3/SLC27A3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP3/SLC27A3 Antibody (A12345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1).
FATP3/SLC27A3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP3/SLC27A3 Antibody (A12345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For SLC27A3 (Source: Uniprot.org, NCBI)

Alternative Names

ACSVL3; EC 6.2.1; EC 6.2.1.-; EC 6.2.1.7; FATP3; FATP-3; FATP3ACSVL3MGC4365; Fatty acid transport protein 3; long-chain fatty acid transport protein 3; SLC27A3; solute carrier family 27 (fatty acid transporter), member 3; Solute carrier family 27 member 3; Very long-chain acyl-CoA synthetase homolog 3; VLCS-3 SLC27A3 ACSVL3, FATP3, VLCS-3 solute carrier family 27 member 3 solute carrier family 27 member 3|arachidonate--CoA ligase|long-chain fatty acid transport protein 3|solute carrier family 27 (fatty acid transporter), member 3|very long-chain acyl-CoA synthetase homolog 3

For more info on SLC27A3, check out the SLC27A3 Infographic

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