|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-Fatty Acid Binding Protein 5 Antibody|
|Description||Rabbit IgG polyclonal antibody for Fatty acid-binding protein, epidermal(FABP5) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Cite This Product||Anti-Fatty Acid Binding Protein 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1853)|
|Replacement Item||This antibody may replace the following items: sc-16060|sc-365166|sc-365236|sc-373788|sc-50379 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of mouse Fatty Acid Binding Protein 5(10-23aa KWRLMESHGFEEYM), different from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Fatty acid-binding protein, epidermal|
|Molecular Weight||15137 MW|
|Protein Function||High specificity for fatty acids. Highest affinity for C18 chain length (By similarity). .|
|Tissue Specificity||Most abundant in keratinocytes and also in stratified epithelia of epidermis and tongue. Relatively high levels found in adipose and mammary tissues and small amounts found in heart, brain, liver, spleen, muscle and lung.|
|Alternative Names||Fatty acid-binding protein, epidermal;Epidermal-type fatty acid-binding protein;E-FABP;Fatty acid-binding protein 5;Keratinocyte lipid-binding protein;Psoriasis-associated fatty acid-binding protein homolog;PA-FABP;Fabp5;Fabpe, Klbp, Mal1;|
|Research Areas|||cardiovascular|lipids / lipoproteins|fatty acids|binding proteins| signal transduction|metabolism|lipid metabolism| cardiovascular| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|cancer|redox metabolism|fatty acid oxidation||
Background for Fatty acid-binding protein, epidermal
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Fatty Acid Binding Protein 5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Fatty Acid Binding Protein 5 Antibody Images
Click the images to enlarge.
All lanes: Anti Fatty Acid Binding Protein 5 (PA1853) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 15KD
Observed bind size: 15KD
IHC(P): Rat Intestine Tissue
IHC(P): Mouse Cardiac Muscle
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,