Anti-Fatty Acid Binding Protein 5/FABP5 Antibody

Product Name

Anti-Fatty Acid Binding Protein 5/FABP5 Antibody

View all FABP5/E-FABP Antibodies

SKU/Catalog Number

PA1853

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fatty Acid Binding Protein 5/FABP5 Antibody catalog # PA1853. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fatty Acid Binding Protein 5/FABP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1853)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Fatty Acid Binding Protein 5, different from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1853 is reactive to FABP5 in Mouse, Rat

Applications

PA1853 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

15.164kDa

Background of FABP5/E-FABP

FABP5 (fatty acid binding protein 5 (psoriasis-associated)) is a protein that in humans is encoded by the FABP5 gene, also known as PAFABP, EFABP, E-FABP, KFABP, PA-FABP, Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog. It is a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. It is though that FABPs roles include fatty acid uptake, transport, and metabolism. Kaczocha et al. (2009) identified FABP5 and FABP7 as cytosolic proteins that transport AEA from the plasma membrane to subcellular fatty acid amide hydrolase, where it is hydrolyzed and inactivated. FABP3 did not show this specific transport function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FABP5 using anti-FABP5 antibody (PA1853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat intestine tissue lysates.
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP5 antigen affinity purified polyclonal antibody (Catalog # PA1853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15 kDa. The expected band size for FABP5 is at 15 kDa.

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Figure 2. IHC analysis of FABP5 using anti-FABP5 antibody (PA1853).
FABP5 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of FABP5 using anti-FABP5 antibody (PA1853).
FABP5 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For FABP5 (Source: Uniprot.org, NCBI)

Alternative Names

EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog FABP5 E-FABP, EFABP, KFABP, PA-FABP, PAFABP fatty acid binding protein 5 fatty acid-binding protein 5|epidermal-type fatty acid-binding protein|fatty acid binding protein 5 (psoriasis-associated)|psoriasis-associated fatty acid-binding protein homolog

For more info on FABP5, check out the FABP5 Infographic

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In this infographic, you will see the following information for FABP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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