Anti-Fbp5B FBXO43 Antibody

Product Name

Anti-Fbp5B FBXO43 Antibody

View all FBXO43 Antibodies

SKU/Catalog Number

A12000

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Fbp5B FBXO43 Antibody (Catalog # A12000). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Chimpanzee.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Fbp5B FBXO43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12000)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa

Reactive Species

A12000 is reactive to FBXO43 in Human, Mouse, Rat, Chimpanzee

Observed Molecular Weight

42 kDa

Calculated molecular weight

78.4 kDa

Background of FBXO43

Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12000 is guaranteed for ELISA, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IP: 1:100
WB: 1:500 - 1:3,000
This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 kDa in size corresponding to Fbp5B protein by western blotting in the appropriate cell lysate or extract.  Predicted molecular weight is 51 kDa.

Validation Images & Assay Conditions

See full target information FBXO43

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