Product Info Summary
SKU: | PB9242 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Fetuin A/AHSG Antibody Picoband™
SKU/Catalog Number
PB9242
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fetuin A/AHSG Antibody Picoband™ catalog # PB9242. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fetuin A/AHSG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9242)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Fetuin A recombinant protein (Position: A19-I345). Mouse Fetuin A shares 57% and 85% amino acid (aa) sequence identity with human and rat Fetuin A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9242 is reactive to AHSG in Mouse
Applications
PB9242 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
39.341kDa
Background of AHSG
Alpha-2-HS-glycoprotein (AHSG) also known as fetuin-A is a protein that in humans is encoded by the AHSG gene. Fetuin-A belongs to the fetuin class of plasma binding proteins and is more abundant in fetal than adult blood. This gene is mapped to 3q27. Fetuin-A is a secreted partially phosphorylated glycoprotein with complex proeolytic processing that circulates in blood and extracellular fluids. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin-A is also a potent inhibitor of pathological calcification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Fetuin A/AHSG using anti-Fetuin A/AHSG antibody (PB9242).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse plasma lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fetuin A/AHSG antigen affinity purified polyclonal antibody (Catalog # PB9242) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fetuin A/AHSG at approximately 55-60 kDa. The expected band size for Fetuin A/AHSG is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For AHSG (Source: Uniprot.org, NCBI)
Gene Name
AHSG
Full Name
Alpha-2-HS-glycoprotein
Weight
39.341kDa
Superfamily
fetuin family
Alternative Names
A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA AHSG A2HS, AHS, APMR1, FETUA, HSGA alpha 2-HS glycoprotein alpha-2-HS-glycoprotein|alpha-2-Z-globulin|ba-alpha-2-glycoprotein|fetuin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AHSG, check out the AHSG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AHSG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fetuin A/AHSG Antibody Picoband™ (PB9242)
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No publications found for PB9242
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