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Product Info Summary
| SKU: | M03460 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-FEV Antibody (N-Term)
SKU/Catalog Number
M03460
Size
50 µl
Description
Boster Bio Anti-FEV Antibody (N-Term) (Catalog # M03460). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FEV Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03460)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FEV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FEV.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03460 is reactive to FEV in Human
Applications
M03460 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.03kDa
Background of Pet1
Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-FEV Antibody (N-Term) at 1:2000 dilution + K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03460 staining FEV in human small intestine sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For FEV (Source: Uniprot.org, NCBI)
Gene Name
FEV
Full Name
Protein FEV
Weight
25.03kDa
Superfamily
ETS family
Alternative Names
FEV (ETS oncogene family); FEV (fifth Ewing variant); Fifth Ewing variant protein; HSRNAFEV; PC12 ETS domain-containing transcription factor 1; PC12 ETS factor 1; PET1; Pet-1; protein FEV FEV HSRNAFEV, PET-1 FEV transcription factor, ETS family member protein FEV|FEV (ETS oncogene family)|FEV, ETS transcription factor|PC12 ETS domain-containing transcription factor 1|fifth Ewing variant protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FEV, check out the FEV Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FEV: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FEV Antibody (N-Term) (M03460)
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No publications found for M03460
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Customer Reviews
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