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Product Info Summary
| SKU: | A02437 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FFAR3/Gpr41 Antibody
SKU/Catalog Number
A02437
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FFAR3/Gpr41 Antibody (Catalog# A02437). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FFAR3/Gpr41 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02437)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human FFAR3. Immunogen sequence location: 22221
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02437 is reactive to FFAR3 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02437 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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Immunofluorescence analysis of LOVO cells, using FFAR3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-FFAR3/Gpr41 Antibody (A02437)
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Customer Q&As
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6 Customer Q&As for Anti-FFAR3/Gpr41 Antibody
Question
Is this A02437 anti-FFAR3/Gpr41 antibody reactive to the isotypes of FFAR3?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The immunogen of A02437 anti-FFAR3/Gpr41 antibody is A peptide derived from human FFAR3. Immunogen sequence location: 22221. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-14
Question
Will anti-FFAR3/Gpr41 antibody A02437 work for IF with brain ovary?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
According to the expression profile of brain ovary, FFAR3 is highly expressed in brain ovary. So, it is likely that anti-FFAR3/Gpr41 antibody A02437 will work for IF with brain ovary.
Boster Scientific Support
Answered: 2018-08-20
Question
See attached the WB image, lot number and protocol we used for brain ovary using anti-FFAR3/Gpr41 antibody A02437. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-18
Question
We are currently using anti-FFAR3/Gpr41 antibody A02437 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
C. Anderson
Verified customer
Asked: 2014-05-01
Answer
The anti-FFAR3/Gpr41 antibody (A02437) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain ovary using anti-FFAR3/Gpr41 antibody A02437. Let me know if you need anything else.
K. Dhar
Verified customer
Asked: 2014-04-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-01
Question
My question regarding product A02437, anti-FFAR3/Gpr41 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Anderson
Verified customer
Asked: 2013-12-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02437 anti-FFAR3/Gpr41 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-10
