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Product Info Summary
| SKU: | M00802 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FGF21 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00802
BM5034 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FGF21 Rabbit Monoclonal Antibody catalog # M00802. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF21 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00802)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AG-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FGF21
Reactive Species
M00802 is reactive to FGF21 in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00802 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of FGF21 expression in (1) HeLa cell lysate; (2) Mouse spleen lysate.
Specific Publications For Anti-FGF21 Rabbit Monoclonal Antibody (M00802)
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Customer Q&As
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16 Customer Q&As for Anti-FGF21 Rabbit Monoclonal Antibody
Question
I see that the anti-FGF21 Rabbit Monoclonal antibody M00802 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-20
Question
My boss were content with the WB result of your anti-FGF21 Rabbit Monoclonal antibody. However we have observed positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
From literature, lung does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-04
Question
We have seen staining in human lung. Do you have any suggestions? Is anti-FGF21 Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
From literature lung does express FGF21. From Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-04
Question
Would M00802 anti-FGF21 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
As indicated on the product datasheet, M00802 anti-FGF21 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-19
Question
Is there a BSA free version of anti-FGF21 Rabbit Monoclonal antibody M00802 available?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF21 Rabbit Monoclonal antibody M00802 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-23
Question
Would anti-FGF21 Rabbit Monoclonal antibody M00802 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
According to the expression profile of lung, FGF21 is highly expressed in lung. So, it is likely that anti-FGF21 Rabbit Monoclonal antibody M00802 will work for WB with lung.
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-FGF21 Rabbit Monoclonal antibody M00802 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
The anti-FGF21 Rabbit Monoclonal antibody (M00802) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-03
Question
We bought anti-FGF21 Rabbit Monoclonal antibody for IHC on lung in a previous project. I am using rat, and I plan to use the antibody for WB next. I am looking for examining lung as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
I looked at the website and datasheets of our anti-FGF21 Rabbit Monoclonal antibody and it appears that M00802 has been tested on rat in both IHC and WB. Thus M00802 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-05
Question
Is a blocking peptide available for product anti-FGF21 Rabbit Monoclonal antibody (M00802)?
J. Thomas
Verified customer
Asked: 2017-03-08
Answer
We do provide the blocking peptide for product anti-FGF21 Rabbit Monoclonal antibody (M00802). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-03-08
Question
Can you help my question with product M00802, anti-FGF21 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Singh
Verified customer
Asked: 2016-11-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00802 anti-FGF21 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-02
Question
I am interested in using your anti-FGF21 Rabbit Monoclonal antibody for negative regulation of endothelial cell apoptotic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
H. Lewis
Verified customer
Asked: 2016-08-31
Answer
I appreciate your inquiry. This M00802 anti-FGF21 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-08-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-FGF21 Rabbit Monoclonal antibody M00802. Let me know if you need anything else.
B. Dhar
Verified customer
Asked: 2015-08-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-19
Question
I am looking for to test anti-FGF21 Rabbit Monoclonal antibody M00802 on mouse lung for research purposes, then I may be interested in using anti-FGF21 Rabbit Monoclonal antibody M00802 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Krishna
Verified customer
Asked: 2014-06-27
Answer
The products we sell, including anti-FGF21 Rabbit Monoclonal antibody M00802, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-27
Question
Is this M00802 anti-FGF21 Rabbit Monoclonal antibody reactive to the isotypes of FGF21?
K. Parker
Verified customer
Asked: 2014-03-10
Answer
The immunogen of M00802 anti-FGF21 Rabbit Monoclonal antibody is A synthesized peptide derived from human FGF21. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-03-10
Question
Please see the WB image, lot number and protocol we used for lung using anti-FGF21 Rabbit Monoclonal antibody M00802. Please let me know if you require anything else.
C. Parker
Verified customer
Asked: 2013-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-09
Question
I was wanting to use your anti-FGF21 Rabbit Monoclonal antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
R. Lewis
Verified customer
Asked: 2013-09-12
Answer
It shows on the product datasheet, M00802 anti-FGF21 Rabbit Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-12
