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Product Info Summary
| SKU: | M00200 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FGFR3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00200
BM5074 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FGFR3 Rabbit Monoclonal Antibody catalog # M00200. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00200)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAID-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FGFR3
Reactive Species
M00200 is reactive to FGFR3 in Human, Mouse, Rat
Observed Molecular Weight
98 kDa
Calculated molecular weight
87.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00200 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of FGFR3 expression in (1) A549 cell lysate; (2) Mouse brain lysate.
Specific Publications For Anti-FGFR3 Rabbit Monoclonal Antibody (M00200)
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6 Customer Q&As for Anti-FGFR3 Rabbit Monoclonal Antibody
Question
Does M00200 anti-FGFR3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
As indicated on the product datasheet, M00200 anti-FGFR3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
I have a question about product M00200, anti-FGFR3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00200 anti-FGFR3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-FGFR3 Rabbit Monoclonal antibody M00200. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-30
Question
I am interested in to test anti-FGFR3 Rabbit Monoclonal antibody M00200 on mouse erythroleukemia for research purposes, then I may be interested in using anti-FGFR3 Rabbit Monoclonal antibody M00200 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
The products we sell, including anti-FGFR3 Rabbit Monoclonal antibody M00200, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-11
Question
Is a blocking peptide available for product anti-FGFR3 Rabbit Monoclonal antibody (M00200)?
S. Carter
Verified customer
Asked: 2016-12-21
Answer
We do provide the blocking peptide for product anti-FGFR3 Rabbit Monoclonal antibody (M00200). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-12-21
Question
Will anti-FGFR3 Rabbit Monoclonal antibody M00200 work for WB with erythroleukemia?
A. Kulkarni
Verified customer
Asked: 2014-04-04
Answer
According to the expression profile of erythroleukemia, FGFR3 is highly expressed in erythroleukemia. So, it is likely that anti-FGFR3 Rabbit Monoclonal antibody M00200 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2014-04-04
