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Product Info Summary
| SKU: | A06303-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FGL2 antibody
SKU/Catalog Number
A06303-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FGL2 antibody catalog # A06303-3. Tested in WB, IHC, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-FGL2 antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06303-3)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human FGL2 recombinant protein (Position: M1-K145)
Reactive Species
A06303-3 is reactive to FGL2 in Human, Mouse, Rat
Calculated molecular weight
50.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06303-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000 Click image to see more details Western blot analysis of FGL2 using anti-FGL2 antibody (A06303-3).
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400Validation Images & Assay Conditions
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGL2 antigen affinity purified polyclonal antibody (A06303-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGL2 at approximately 85 kDa. The expected band size for FGL2 is at 50 kDa.
Specific Publications For Anti-FGL2 antibody (A06303-3)
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Customer Reviews
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1 Reviews For Anti-FGL2 antibody
Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.
Excellent
| SKU | A06303-3 |
|---|---|
| Application | Western Blot |
| Sample | rat enteric glial cells and mouse tissue |
| Sample Processing Description | Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min. |
| Primary Incubation | The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 2 hour |
| Other Reagents used | 5% non-fat milk |
| Detection | Signal was developed using ECL substrate on an ImageQuant LAS4000mini system. |
| Results Summary | Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands. |
Xing Yang, Institute of Materia Medica, Chinese Academy of Medical Sciences
Verified customer
Submitted 2025-09-23
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