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Product Info Summary
| SKU: | A06303-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-FGL2 Antibody Picoband®
SKU/Catalog Number
A06303-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGL2 Antibody Picoband® catalog # A06303-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FGL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06303-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human FGL2 recombinant protein (Position: N25-E245).
Reactive Species
A06303-3 is reactive to FGL2 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
50.2 kDa
Background of FGL2
The protein encoded by this gene is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06303-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human |
| Flow Cytometry (Fixed) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of FGL2 using anti-FGL2 antibody (A06303-3).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGL2 antigen affinity purified polyclonal antibody (A06303-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGL2 at approximately 60 kDa. The expected band size for FGL2 is at 50 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-FGL2 antibody (A06303-3).
Overlay histogram showing HepG2 cells stained with A06303-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGL2 Antibody (A06303-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-FGL2 Antibody Picoband® (A06303-3)
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Customer Reviews
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1 Reviews For Anti-FGL2 Antibody Picoband®
Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands.
Excellent
| SKU | A06303-3 |
|---|---|
| Application | Western Blot |
| Sample | rat enteric glial cells and mouse tissue |
| Sample Processing Description | Samples (5 µL per lane) were run on 10% resolving and stacking gels at 80 V for 20 min, then 150 V for 70 min, and transferred at 400 mA for 100 min. |
| Primary Incubation | The membrane was incubated with the FGL-2 primary antibody (1:1000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 2 hour |
| Other Reagents used | 5% non-fat milk |
| Detection | Signal was developed using ECL substrate on an ImageQuant LAS4000mini system. |
| Results Summary | Works very well in Western blot for rat enteric glial cells and mouse FGL-2 protein, with only slight nonspecific bands. |
Xing Yang, Institute of Materia Medica, Chinese Academy of Medical Sciences
Verified customer
Submitted 2025-09-23
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