Anti-Fibronectin FN1 Antibody Biotin Conjugated

Product Name

Anti-Fibronectin FN1 Antibody Biotin Conjugated

View all FN1 Antibodies

SKU/Catalog Number

B00564

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Fibronectin FN1 Antibody Biotin Conjugated (Catalog # B00564). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Monkey.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Fibronectin FN1 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00564)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B00564 is reactive to FN1 in Bovine, Human, Monkey, Mouse, Rat

Observed Molecular Weight

42 kDa

Calculated molecular weight

272.3 kDa

Background of FN1

Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

B00564 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
Fluorochrome Protein Value: 10-20
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:5,000
Anti-Fibronectin (rabbit) antibody was tested by WB, ELISA, and IHC. Assay by immunoblot was found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000.  Assay against 1.0 µg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.   A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product.  For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.

Validation Images & Assay Conditions

See full target information FN1

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