Click image to see more details
Product Info Summary
| SKU: | M04492 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FKBP12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04492
BM5048 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FKBP12 Rabbit Monoclonal Antibody catalog # M04492. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FKBP12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04492)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAFH-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FKBP12
Reactive Species
M04492 is reactive to FKBP1A in Human, Mouse, Rat
Observed Molecular Weight
12 kDa
Calculated molecular weight
12.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04492 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human PC-3 whole cell, human HepG2 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FKBP1A using anti-FKBP1A antibody (M04492).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP1A antigen affinity purified monoclonal antibody (M04492) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FKBP1A at approximately 12 kDa. The expected band size for FKBP1A is at 12 kDa.
Specific Publications For Anti-FKBP12 Rabbit Monoclonal Antibody (M04492)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FKBP12 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-FKBP12 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-FKBP12 Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-FKBP12 Rabbit Monoclonal antibody M04492 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FKBP12 Rabbit Monoclonal antibody M04492 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
Please see the WB image, lot number and protocol we used for caudate nucleus using anti-FKBP12 Rabbit Monoclonal antibody M04492. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
My question regards to test anti-FKBP12 Rabbit Monoclonal antibody M04492 on mouse caudate nucleus for research purposes, then I may be interested in using anti-FKBP12 Rabbit Monoclonal antibody M04492 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The products we sell, including anti-FKBP12 Rabbit Monoclonal antibody M04492, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-11
Question
Can you help my question with product M04492, anti-FKBP12 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Jones
Verified customer
Asked: 2018-08-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04492 anti-FKBP12 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-28
Question
We are currently using anti-FKBP12 Rabbit Monoclonal antibody M04492 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
The anti-FKBP12 Rabbit Monoclonal antibody (M04492) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-23
Question
Does anti-FKBP12 Rabbit Monoclonal antibody M04492 work for IF with caudate nucleus?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
According to the expression profile of caudate nucleus, FKBP1A is highly expressed in caudate nucleus. So, it is likely that anti-FKBP12 Rabbit Monoclonal antibody M04492 will work for IF with caudate nucleus.
Boster Scientific Support
Answered: 2017-10-12
Question
Is this M04492 anti-FKBP12 Rabbit Monoclonal antibody reactive to the isotypes of FKBP1A?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
The immunogen of M04492 anti-FKBP12 Rabbit Monoclonal antibody is A synthesized peptide derived from human FKBP12. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-23
