Anti-FKBP51 Monoclonal Antibody

Product Name

Anti-FKBP51 Monoclonal Antibody

View all FKBP51/FKBP5 Antibodies

SKU/Catalog Number

M04182-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-FKBP51 Monoclonal Antibody catalog # M04182-1. Tested in IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FKBP51 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04182-1)

Host

Mouse

Contents

PBS, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

Hi51B

Isotype

IgG1

Immunogen

Synthetic peptide corresponding to the residues of human FKBP51

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~51kDa.

Reactive Species

M04182-1 is reactive to FKBP5 in Dog, Hamster, Human, Mouse, Rabbit, Rat

Applications

M04182-1 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

51.212kDa

Background of FKBP51/FKBP5

HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:2000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B (M04182-1) . Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody (M04182-1) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red) . Counterstain: DAPI (blue) nuclear stain. Cells stained red. Courtesy of: the Hospital Henri Mondor, France.

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Figure 2. Western blot analysis of FKBP5 using anti-FKBP5 antibody (M04182-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP5 antigen affinity purified polyclonal antibody (Catalog # M04182-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for FKBP5.

Gene/Protein Information For FKBP5 (Source: Uniprot.org, NCBI)

Alternative Names

51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; AIG6,51 kDa FK506-binding protein; Androgen-regulated protein 6; Dit1; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP5; FKBP-5; FKBP51; FKBP-51; FKBP51PPIase FKBP5; FKBP54; FKBP54EC 5.2.1.8; HSP90-binding immunophilin; MGC111006; p54; P54,51 kDa FK506-binding protein 5; peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase; Ptg-10; rotamase; T-cell FK506-binding protein FKBP5 AIG61, FKBP54, P54, PPIase, Ptg-10, FKBP5 FKBP prolyl isomerase 5 peptidyl-prolyl cis-trans isomerase FKBP5|51 kDa FK506-binding protein|51 kDa FKBP|54 kDa progesterone receptor-associated immunophilin|FF1 antigen|FK506 binding protein 5|FKBP-51|HSP90-binding immunophilin|PPIase FKBP5|T-cell FK506-binding protein|androgen-regulated protein 6|peptidylprolyl cis-trans isomerase|rotamase

For more info on FKBP5, check out the FKBP5 Infographic

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In this infographic, you will see the following information for FKBP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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