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Product Info Summary
| SKU: | M30971-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Flag-Tag Mouse Monoclonal Antibody
SKU/Catalog Number
M30971-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Flag-Tag Mouse Monoclonal Antibody catalog # M30971-2. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flag-Tag Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30971-2)
Host
Mouse
Contents
Mouse IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17F08
Isotype
IgG1
Immunogen
A synthesized peptide
Reactive Species
M30971-2 is reactive to #N/A in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30971-2 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Recommend Dilution
WB 1:3000-30000
ICC/IF 1:50-200
IP 1:100
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate.
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Nr5a2 ablation impedes mouse blastocyst formation. Related to Figures 1. A. Evaluation of interference efficiency of SI1 and SI2 assessed by qPCR at the 4-cell stage. Three
repetitions were conducted, data are represented as mean ± SEM.***P < 0.001, ****P < 0.0001. B. Embryo development in response to injection of control interference fragment (NC), SI2 interference
fragment (SI2) at the zygotic stage, respectively, Scale bar: 100 μm.. C. Rate of embryo development at each stage in NC, SI1, and SI2 groups. data are represented as mean ±
SEM. D. Schematic representation of the mutant (Nr5a2*) obtained through synonymous codon mutation of
Nr5a2. E. Verification of overexpression of Flag-labeled Nr5a2 using Western blot analysis. F. Confirmation of overexpression of Flag-labeled Nr5a2 mutant variants using Western blot analysis
Index in PubMed under a CC BY license. PMID: 38386558
Specific Publications For Anti-Flag-Tag Mouse Monoclonal Antibody (M30971-2)
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