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- Table of Contents
CFLAR antibody (A01295-2) performs well in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.
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Anti-FLIP CFLAR Antibody
CFLAR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-FLIP CFLAR Antibody
View all CFLAR Antibodies
View all CFLAR Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
FLIP antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
FLIP Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.
Cross-Reactivity
FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
55.3 kDa
Background of CFLAR
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
Cite This Product
Anti-FLIP CFLAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01295-2)
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Product Promise
We promise Anti-FLIP CFLAR Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01295-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026