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Product Info Summary
| SKU: | A01295 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-FLIP CFLAR Antibody
SKU/Catalog Number
A01295
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FLIP CFLAR Antibody (Catalog # A01295). Tested in ELISA, WB, ICC applications. This antibody reacts with Mouse.
Storage & Handling
FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FLIP CFLAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01295)
Host
Rabbit
Contents
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.
Cross-reactivity
FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Reactive Species
A01295 is reactive to CFLAR in Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.3 kDa
Background of CFLAR
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01295 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.
Specific Publications For Anti-FLIP CFLAR Antibody (A01295)
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