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Product Info Summary
| SKU: | M12068 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-FN3KRP Antibody (N-Term)
SKU/Catalog Number
M12068
Size
80 µl
Description
Boster Bio Anti-FN3KRP Antibody (N-Term) (Catalog # M12068). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FN3KRP Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12068)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FN3KRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from human FN3KRP.
Reactive Species
M12068 is reactive to FN3KRP in Human, Mouse
Calculated molecular weight
34.4 kDa
Background of FN3KRP
Phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of the sugar moiety. Protein- bound psicosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions. Thus phosphorylation leads to deglycation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12068 is guaranteed for IHC-P, WB Boster Guarantee
Recommend Dilution
WB: 1:1000-1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-FN3KRP Antibody (N-Term) at 1:1000-1:2000 dilution
Lane 1: U-87 MG whole cell lysates
Lane 2: 293T-17 whole cell lysates
Lane 3: NCI-H1299 whole cell lysates
Lane 4: Jurkat whole cell lysates
Lane 5: mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 34 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M12068 staining FN3KRP in human Stomach sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-FN3KRP Antibody (N-Term) (M12068)
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