Anti-FNDC5 Antibody Picoband™

Product Name

Anti-FNDC5 Antibody Picoband™

View all Irisin/FNDC5 Antibodies

SKU/Catalog Number

A02538-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FNDC5 Antibody Picoband™ catalog # A02538-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FNDC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02538-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human FNDC5 recombinant protein (Position: D32-E143).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02538-1 is reactive to FNDC5 in Human, Mouse, Rat

Applications

A02538-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.659kDa

Background of Irisin/FNDC5

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.

Gene/Protein Information For FNDC5 (Source: Uniprot.org, NCBI)

Alternative Names

fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin FNDC5 FRCP2, irisin fibronectin type III domain containing 5 fibronectin type III domain-containing protein 5|fibronectin type III repeat-containing protein 2

For more info on FNDC5, check out the FNDC5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FNDC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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