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Product Info Summary
| SKU: | A00011 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody
SKU/Catalog Number
A00011
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody (Catalog # A00011). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FOXP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00011)
Host
Rabbit
Contents
FOXP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal FOXP3 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FOXP3. The immunogen is located within the last 50 amino acids of FOXP3.
Reactive Species
A00011 is reactive to FOXP3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
47.2 kDa
Background of FOXP3
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00011 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FOXP3 antibody can be used for detection of FOXP3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of FOXP3 in human brain tissue with FOXP3 antibody at 5 μg/mL.
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Immunofluorescence of FOXP3 in human brain tissue with FOXP3 antibody at 20 μg/mL.
Green: FOXP3 Antibody (A00011)
Blue: DAPI staining
Specific Publications For Anti-Forkhead box protein P3 FOXP3 Antibody (A00011)
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