Product Info Summary
SKU: | M01819 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-FoxO4/Afx Rabbit Monoclonal Antibody
SKU/Catalog Number
M01819
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FoxO4/Afx Rabbit Monoclonal Antibody catalog # M01819. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FoxO4/Afx Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01819)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HEI-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FoxO4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01819 is reactive to FOXO4 in Human
Applications
M01819 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
53.684kDa
Background of FOXO4
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FoxO4 expression in 293T cell lysate transfected with FoxO4.
Protein Target Info & Infographic
Gene/Protein Information For FOXO4 (Source: Uniprot.org, NCBI)
Gene Name
FOXO4
Full Name
Forkhead box protein O4
Weight
53.684kDa
Alternative Names
AFX; AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7; Fork head domain transcription factor AFX1; forkhead box O4; forkhead box protein O4; MLLT7MGC120490; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7 FOXO4 AFX, AFX1, MLLT7 forkhead box O4 forkhead box protein O4|fork head domain transcription factor AFX1|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXO4, check out the FOXO4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXO4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FoxO4/Afx Rabbit Monoclonal Antibody (M01819)
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No publications found for M01819
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Customer Q&As
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3 Customer Q&As for Anti-FoxO4/Afx Rabbit Monoclonal Antibody
Question
Can you help my question with product M01819, anti-FoxO4/Afx Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use your anti-FoxO4/Afx Rabbit Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Would M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-24