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Product Info Summary
| SKU: | M01819 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FoxO4/Afx Rabbit Monoclonal Antibody
SKU/Catalog Number
M01819
BM4729 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FoxO4/Afx Rabbit Monoclonal Antibody catalog # M01819. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FoxO4/Afx Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01819)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HEI-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FoxO4
Reactive Species
M01819 is reactive to FOXO4 in Human
Observed Molecular Weight
65 kDa
Calculated molecular weight
53.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01819 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of FoxO4 expression in 293T cell lysate transfected with FoxO4.
Specific Publications For Anti-FoxO4/Afx Rabbit Monoclonal Antibody (M01819)
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3 Customer Q&As for Anti-FoxO4/Afx Rabbit Monoclonal Antibody
Question
Can you help my question with product M01819, anti-FoxO4/Afx Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use your anti-FoxO4/Afx Rabbit Monoclonal antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-13
Question
Would M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, M01819 anti-FoxO4/Afx Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-24
