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Product Info Summary
| SKU: | A07915 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-FREM2 Antibody
SKU/Catalog Number
A07915
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FREM2 Antibody (Catalog # A07915). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
FREM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FREM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07915)
Host
Rabbit
Contents
FREM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FREM2 antibody was raised against a 19 amino acid synthetic peptide near the center of human FREM2. The immunogen is located within amino acids 1920 - 1970 of FREM2.
Reactive Species
A07915 is reactive to FREM2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
351.2 kDa
Background of FREM2
FREM2 is a member of the FRAS1-related extracellular matrix protein family and like FREM1, mutations in the FREM2 gene are associated with Fraser syndrome. FREM2 localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions during morphogenetic processes and is thought to be required for maintaining the integrity of the skin epithelium and the differentiated state of renal epithelia. The FREM2 gene is one of several genes whose transcription is affecteded by TFAP2C, a transcription factor involved in mammary development, differentiation, and oncogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07915 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.
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Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.
Specific Publications For Anti-FREM2 Antibody (A07915)
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