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Product Info Summary
| SKU: | A04391 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Frizzled-7 FZD7 Antibody
SKU/Catalog Number
A04391
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Frizzled-7 FZD7 Antibody catalog # A04391. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Frizzled-7 FZD7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04391)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94
Reactive Species
A04391 is reactive to FZD7 in Human, Mouse
Calculated molecular weight
63.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04391 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:50-200
IF/ICC1:100-500
ELISA 1:5000-20000
Validation Images & Assay Conditions
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Western Blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from HT-29 cells, using FZD7 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
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Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Frizzled-7 FZD7 Antibody (A04391)
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