Product Info Summary
SKU: | A06680-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Frizzled-8 (K528) FZD8 Antibody
View all Frizzled-8 Antibodies
SKU/Catalog Number
A06680-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Frizzled-8 (K528) FZD8 Antibody catalog # A06680-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Frizzled-8 (K528) FZD8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06680-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human AMPD3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06680-1 is reactive to FZD8 in Human, Mouse, Rat
Applications
A06680-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
73.3kDa
Background of Frizzled-8
The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons.
Briggs J.A., J. Cell. Biochem. 49:82-92(1992).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Burrus G.R., J. Cell. Biochem. 48:190-202(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of FZD8 using Anti-Frizzled-8 (K528) FZD8 Antibody (A06680-1).
Immunohistochemistry (IHC) analyzes of Frizzled-8 (K528) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 3. Immunohistochemistry validation of FZD8 using Anti-Frizzled-8 (K528) FZD8 Antibody (A06680-1).
Immunohistochemistry (IHC) analyzes of Frizzled-8 (K528) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 1. Western blotting validation for Anti-Frizzled-8 (K528) FZD8 Antibody A06680-1
Western blot (WB) analysis of Frizzled-8 (K528) polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:H1792 whole cell lysate(40ug)
Lane4:COS-7 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For FZD8 (Source: Uniprot.org, NCBI)
Gene Name
FZD8
Full Name
Frizzled-8
Weight
73.3kDa
Superfamily
G-protein coupled receptor Fz/Smo family
Alternative Names
frizzled (Drosophila) homolog 8; frizzled 8; frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8 FZD8 FZ-8, hFZ8 frizzled class receptor 8 frizzled-8|frizzled 8, seven transmembrane spanning receptor|frizzled family receptor 8|frizzled homolog 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FZD8, check out the FZD8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FZD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Frizzled-8 (K528) FZD8 Antibody (A06680-1)
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No publications found for A06680-1
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Customer Reviews
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