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Product Info Summary
| SKU: | PB9594 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Frizzled homolog 1/FZD1 Antibody Picoband®
SKU/Catalog Number
PB9594
PB0625 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Frizzled homolog 1/FZD1 Antibody Picoband® catalog # PB9594. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Frizzled homolog 1/FZD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9594)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Frizzled homolog 1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9594 is reactive to FZD1 in Human
Observed Molecular Weight
71 kDa
Calculated molecular weight
71.2 kDa
Background of FZD1
Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9594 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human
Western blot, 0.1-0.6μg/ml, Human
Positive Control
WB: 22RV1 Whole Cell, 293T Whole Cell
IHC: human prostatic cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Frizzled homolog 1 using anti-Frizzled homolog 1 antibody (PB9594).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: 22RV1 Whole Cell Lysate at 40ug,
Lane 2: 293T Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled homolog 1 antigen affinity purified polyclonal antibody (Catalog # PB9594) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Frizzled homolog 1 at approximately 71 kDa. The expected band size for Frizzled homolog 1 is at 71 kDa.
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IHC analysis of Frizzled homolog 1 using anti-Frizzled homolog 1 antibody (PB9594).
Frizzled homolog 1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Frizzled homolog 1 Antibody (PB9594) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Frizzled homolog 1/FZD1 Antibody Picoband® (PB9594)
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1 Customer Q&As for Anti-Frizzled homolog 1/FZD1 Antibody Picoband®
Question
We are currently using anti-Frizzled homolog 1/FZD1 antibody PB9594 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The anti-Frizzled homolog 1/FZD1 antibody (PB9594) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-02
