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Product Info Summary
| SKU: | DZ41314 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Fruit fly Drp1 Antibody
SKU/Catalog Number
DZ41314
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-Drp1 Antibody catalog # DZ41314. This antibody reacts with Fruit fly.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Fruit fly Drp1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41314)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41314 is reactive to Drp1 in Fruit fly
Application & Images
Applications
DZ41314 is guaranteed for IF, WB Boster Guarantee
Recommend Dilution
WB: 1:1000
IF: 1:100
Validation Images & Assay Conditions
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IF analysis of Drp1 using anti-Drp1 antibody (DZ41314).
Drp1 was detected in a paraffin-embedded section of Drosophila Fat body tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Drp1 Antibody (DZ41314) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.
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The figure shows a Western blot of Drosophila ovaries stained for Drp1 in a Gal4 control line and a Drp1 RNAi. A reduction in Drp1 levels is observed at ~85 kDa in the RNAi sample, as indicated by the molecular weight ladder. β-Actin was used as a loading control.
Specific Publications For Anti-Fruit fly Drp1 Antibody (DZ41314)
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Customer Reviews
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2 Reviews For Anti-Fruit fly Drp1 Antibody
Antibody Optimized and validated
Excellent
| SKU | DZ41314 |
|---|---|
| Application | Western Blot |
| Sample | Drosophila Ovaries |
| Sample Processing Description | Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting |
| Primary Antibody | Anti-Fruit Fly Drp1 Antibody |
| Primary Incubation | 1:1000 in 5% BSA and 0.1% PBST Either 2 hours at RT or Overnight at 4 Degrees |
| Secondary Antibody | 1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated |
| Secondary Incubation | 1 hour at room temperature |
| Other Reagents used | ECL Clarity Substrate and enzyme for Chemiluminescence |
| Detection | Biorad ChemiDoc |
| Results Summary | In Drosophila ovaries, loss of Drp1 function was confirmed. Western blot analysis validated Drp1 knockdown, showing reduced Drp1 protein levels (~85 kDa) in ovaries expressing Drp1 miRNA, with β-Actin serving as a loading control. |
Kasturi Mitra
Verified customer
Submitted 2026-01-06
Antibody Optimized and validated
Excellent
| SKU | DZ41314 |
|---|---|
| Application | Immunofluorescence |
| Sample | Drosophila Fat body |
| Sample Processing Description | Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes, Secondary, Washes, Nuclei Staining, Slide Preparation |
| Primary Antibody | Anti-Fruit Fly Drp1 Antibody |
| Primary Incubation | 1:100 in 2%BSA and 0.1% PBS-TX for Fat body |
| Secondary Antibody | 1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 |
| Secondary Incubation | 1 hour at room temperature |
| Detection | Zeiss LSM 900 Confocal Microscope with Airyscan 2 |
| Results Summary | In Drosophila fatbody, Drp1 was seen as punctate structures, as expected from the literature on mitochondria. |
Kasturi Mitra
Verified customer
Submitted 2026-01-06
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