Product Info Summary
| SKU: | A15981 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-FXYD7 Antibody
SKU/Catalog Number
A15981
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FXYD7 Antibody (Catalog # A15981). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FXYD7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FXYD7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15981)
Host
Rabbit
Contents
FXYD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FXYD7 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human FXYD7. The immunogen is located within the first 50 amino acids of FXYD7.
Cross-reactivity
FXYD7 antibody is human, mouse and rat reactive. FXYDY7 antibody is predicted to not react with other members of the FXDY protein family
Reactive Species
A15981 is reactive to FXYD7 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
8.5 kDa
Background of FXYD7
FXYD7 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing seven invariant and six highly conserved amino acids. The FXYD proteins are tissue-specific regulators of Na, K-ATPase, with FXYD7 initially identified as a brain-specific member. FXYD7 interacts with Na, K-ATPase through its transmembrane domain and is thought to influence the affinity of Na, K-ATPase for external K+ and Na+ ions. Other members of the FXDY family have similar functions: FXYD2 regulates the properties of Na, K-ATPase, while FXYD1 (phospholemman), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15981 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FXYD7 antibody can be used for detection of FXYD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FXYD7 in human lung tissue lysate with FXYD7 antibody at 1 μg/mL.
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Immunohistochemistry of FXYD7 in rat lung tissue with FXYD7 antibody at 2.5 μg/mL.
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Immunofluorescence of FXYD4 in rat lung tissue with FXYD4 antibody at 20 μg/mL.
Specific Publications For Anti-FXYD7 Antibody (A15981)
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