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Product Info Summary
| SKU: | M00684-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-FYN Purified Monoclonal Antibody
SKU/Catalog Number
M00684-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FYN Purified Monoclonal Antibody (Catalog# M00684-1). Tested in IP, WB, IHC-P, ICC application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-FYN Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00684-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
FYN-01
Isotype
Mouse IgG2b
Immunogen
Bacterially expressed recombinant fragment of human Fyn (aa 7-176). The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
Reactive Species
M00684-1 is reactive to FYN in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
60.8 kDa
Background of FYN
Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00684-1 is guaranteed for IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: brain.
Western blotting: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line.
Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Validation Images & Assay Conditions
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Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01).
Lane 1: original lysate of T cells
Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.
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Immunocytochemistry analysis of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Primary antibody: 5 ug/ml
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Immunocytochemistry analysis of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Specific Publications For Anti-FYN Purified Monoclonal Antibody (M00684-1)
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