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Product Info Summary
| SKU: | M01989 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-GAB1 Monoclonal Antibody
SKU/Catalog Number
M01989
BM5737 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAB1 Monoclonal Antibody catalog # M01989. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01989)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFEF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAB1 Probably involved in EGF and insulin receptor signaling.
Reactive Species
M01989 is reactive to GAB1 in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01989 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:500
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of GAB1 expression in HEK293 cell lysate.
Specific Publications For Anti-GAB1 Monoclonal Antibody (M01989)
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Customer Q&As
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7 Customer Q&As for Anti-GAB1 Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-GAB1 Monoclonal antibody M01989. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
Do you have a BSA free version of anti-GAB1 Monoclonal antibody M01989 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAB1 Monoclonal antibody M01989 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
What sample was tested in WB and IHC applications with M01989?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of GAB1 expression in HEK293 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-GAB1 Monoclonal antibody for IF for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
You can see on the product datasheet, M01989 anti-GAB1 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-05
Question
Will M01989 anti-GAB1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
It shows on the product datasheet, M01989 anti-GAB1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-09
Question
I am interested in to test anti-GAB1 Monoclonal antibody M01989 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-GAB1 Monoclonal antibody M01989 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
The products we sell, including anti-GAB1 Monoclonal antibody M01989, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-10
Question
Is this M01989 anti-GAB1 Monoclonal antibody reactive to the isotypes of GAB1?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
The immunogen of M01989 anti-GAB1 Monoclonal antibody is A synthesized peptide derived from human GAB1 Probably involved in EGF and insulin receptor signaling. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-23
