Product Info Summary
SKU: | A02386-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GAB2 Antibody Picoband™
SKU/Catalog Number
A02386-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAB2 Antibody Picoband™ catalog # A02386-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GAB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02386-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GAB2 recombinant protein (Position: S162-R504).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02386-3 is reactive to GAB2 in Human, Mouse
Applications
A02386-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
74.458kDa
Background of GAB2
GRB2-associated-binding protein 2 also known as GAB2 is a protein that in humans is encoded by the GAB2 gene. This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Rat
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GAB2 using anti-GAB2 antibody (A02386-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAB2 antigen affinity purified polyclonal antibody (Catalog # A02386-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAB2 at approximately 90 kDa. The expected band size for GAB2 is at 74 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HL-60 cells using anti-GAB2 antibody (A02386-3).
Overlay histogram showing HL-60 cells stained with A02386-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of HL-60 cells using anti-GAB2 antibody (A02386-3).
Overlay histogram showing HL-60 cells stained with A02386-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Flow Cytometry analysis of THP-1 cells using anti-GAB2 antibody (A02386-3).
Overlay histogram showing THP-1 cells stained with A02386-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For GAB2 (Source: Uniprot.org, NCBI)
Gene Name
GAB2
Full Name
GRB2-associated-binding protein 2
Weight
74.458kDa
Superfamily
GAB family
Alternative Names
GAB2; GRB2-associated binding protein 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571GRB2-associated binder 2; p100; pp100 GAB2 GRB2 associated binding protein 2 GRB2-associated-binding protein 2|Grb2-associated binder 2|growth factor receptor bound protein 2-associated protein 2|pp100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAB2, check out the GAB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAB2 Antibody Picoband™ (A02386-3)
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