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Product Info Summary
| SKU: | M01907 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GABARAP Monoclonal Antibody
SKU/Catalog Number
M01907
BM5326 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GABARAP Monoclonal Antibody catalog # M01907. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABARAP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01907)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACHE-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GABARAP GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.
Reactive Species
M01907 is reactive to GABARAP in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
13.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01907 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-10000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human U87 whole cell, human U251 whole cell, human HepG2 whole cell, human HUH7 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GABARAP using anti-GABARAP antibody (M01907).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUH7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified monoclonal antibody (Catalog # M01907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18 kDa. The expected band size for GABARAP is at 14 kDa.
Specific Publications For Anti-GABARAP Monoclonal Antibody (M01907)
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3 Customer Q&As for Anti-GABARAP Monoclonal Antibody
Question
I see that the anti-GABARAP Monoclonal antibody M01907 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-20
Question
Does M01907 anti-GABARAP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Johnson
Verified customer
Asked: 2017-09-21
Answer
As indicated on the product datasheet, M01907 anti-GABARAP Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-21
Question
We are currently using anti-GABARAP Monoclonal antibody M01907 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Collins
Verified customer
Asked: 2016-08-11
Answer
The anti-GABARAP Monoclonal antibody (M01907) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-11
