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Product Info Summary
| SKU: | A05350 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GABP- alpha Monoclonal Antibody
SKU/Catalog Number
A05350
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GABP- alpha Monoclonal Antibody catalog # A05350. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GABP- alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05350)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8C1B1
Isotype
IgG
Immunogen
Purified recombinant fragment of human GABP-α (aa120-190) expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05350 is reactive to GABPA in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
51.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05350 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Confocal immunofluorescence analysis of Hela cells using GABP-α Monoclonal Antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Western Blot analysis using GABP-α Monoclonal Antibody against HeLa (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.
Specific Publications For Anti-GABP- alpha Monoclonal Antibody (A05350)
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Customer Q&As
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6 Customer Q&As for Anti-GABP- alpha Monoclonal Antibody
Question
We are currently using anti-GABP- alpha Monoclonal antibody A05350 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The anti-GABP- alpha Monoclonal antibody (A05350) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-GABP- alpha Monoclonal antibody A05350 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-17
Question
Will A05350 anti-GABP- alpha Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
It shows on the product datasheet, A05350 anti-GABP- alpha Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
I am looking for to test anti-GABP- alpha Monoclonal antibody A05350 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-GABP- alpha Monoclonal antibody A05350 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Jha
Verified customer
Asked: 2018-09-14
Answer
The products we sell, including anti-GABP- alpha Monoclonal antibody A05350, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-14
Question
Will anti-GABP- alpha Monoclonal antibody A05350 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
According to the expression profile of cervix carcinoma, GABPA is highly expressed in cervix carcinoma. So, it is likely that anti-GABP- alpha Monoclonal antibody A05350 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-09-14
Question
Is there a BSA free version of anti-GABP- alpha Monoclonal antibody A05350 available?
H. Moore
Verified customer
Asked: 2016-07-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GABP- alpha Monoclonal antibody A05350 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-05
